RESUMO
Viral satellite RNAs (satRNAs) are small subviral particles that are associated with the genomic RNA of a helper virus (HV). Their replication, encapsidation, and movement depend on the HV. In this paper, we performed a global analysis of the satRNAs associated with different isolates of tomato black ring virus (TBRV). We checked the presence of satRNAs in 42 samples infected with TBRV, performed recombination and genetic diversity analyses, and examined the selective pressure affecting the satRNAs population. We identified 18 satRNAs in total that differed in length and the presence of point mutations. Moreover, we observed a strong effect of selection operating upon the satRNA population. We also constructed infectious cDNA clones of satRNA and examined the viral load of different TBRV isolates in the presence and absence of satRNAs, as well as the accumulation of satRNA molecules on infected plants. Our data provide evidence that the presence of satRNAs significantly affects viral load; however, the magnitude of this effect differs among viral isolates and plant hosts. We also showed a positive correlation between the number of viral genomic RNAs (gRNAs) and satRNAs for two analysed TBRV isolates.
Assuntos
RNA Satélite , RNA Viral , Variação Genética , Vírus Auxiliares/genética , Nepovirus , Doenças das Plantas/genética , Plantas/genética , RNA Satélite/genética , RNA Viral/genética , Replicação Viral/genéticaRESUMO
The search for different life forms elsewhere in the universe is a fascinating area of research in astrophysics and astrobiology. Currently, according to the NASA Exoplanet Archive database, 3876 exoplanets have been discovered. The Earth Similarity Index (ESI) is defined as the geometric mean of radius, density, escape velocity, and surface temperature and ranges from 0 (dissimilar to Earth) to 1 (similar to Earth). The ESI was created to index exoplanets on the basis of their similarity to Earth. In this paper, we examined rocky exoplanets whose physical conditions are potentially suitable for the survival of rock-dependent extremophiles, such as the cyanobacteria Chroococcidiopsis and the lichen Acarospora. The Rock Similarity Index (RSI) is first introduced and then applied to 1659 rocky exoplanets. The RSI represents a measure for Earth-like planets on which physical conditions are potentially suitable for rocky extremophiles that can survive in Earth-like extreme habitats (i.e., hot deserts and cold, frozen lands).
